Development, Validation and Application of a Spectrofluorimetric Method for the Quantification of Nevirapine in Pharmaceutical Formulations Tablets and Suspensions

The development of the spectrofluorimetric method can be considered a promising alternative that is relatively less expensive and sufficiently reliable. In current literature, no for analysis nevirapine by spectro-fluorimetric has been reported. proposed based on transformation naturally non-fluorescent into fluorescent derivative after chemical synthesis. Maximum excitation emission wavelengths are 290 nm 357 respectively. analytical performance demonstrates linearity in concentration range 1.5 × 10-2 13.5 μg/mL with correlation coefficient (r) greater than 0.999. detection (LOD) quantification (LOQ) limits found 1.97 10-3 5.48 Recovery achieved 99.9% 100.3% trueness, intra-day precision variation repeatability (CVr) 0.99% inter-day (CVR) 1.7%. successfully applied 10 batches tablets suspensions.